Profil Institute uses a variety of state-of-the-art methods to perform complex metabolic research to understand and describe the mechanism of action of a given compound or to demonstrate the mechanistic concept behind diabetes or obesity drugs in development.
Profil Institute’s cardiometabolic research methods include:
- Automated glucose clamps, including euglycemic, hyperglycemic, hypoglycemic and pancreatic clamps
- Body composition measurements: DEXA, bioimpedance, doubly labeled water
- Cognitive function testing and mood assessment
- Endogenous glucose production and turnover (tracer methods)
- Energy expenditure: Indirect calorimetry, doubly labeled water
- Insulin sensitivity measurements (glucose clamp and non-glucose clamp techniques)
- Food intake measurements
- Islet cell function assessment, including graded glucose infusions
- Magnetic resonance imaging (MRI)
- Magnetic resonance spectroscopy (MRS)
- Metabolic pathway biomarkers
- Oral and intravenous glucose tolerance tests
- Research nutrition: precise meal planning and delivery
- Satiety, appetite and hunger assessments
- Standardized meal challenge testing
- Tissue biopsies and tissue culture
There is often a need to address overlapping aspects of these closely related conditions. This can be accomplished by combining investigative methods as appropriate.
Beyond the methods that are specific for metabolic research questions, Profil Institute uses all standard research tools as they are needed in an early phase clinical research.
- 24-hour blood pressure and holter monitoring
- Cardiac telemetry
- Extensive range of imaging, including CT, MRI, MRS and PET scanning
- Fundus photography
Profil Institute is a leading institute in performing glucose clamp studies, globally renowned as the gold standard for the evaluation of the blood glucose-lowering action of anti-diabetic agents and determination of their time action profiles. Our annual number of glucose clamp experiments exceeds 1,000 with the ability to conduct 15+ automated clamps in parallel. Profil Institute conducts euglycemic, hypoglycemic and hyperglycemic glucose clamps together with complementary investigative techniques, such as isotopic determination of glucose kinetics, as required.